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Human parainfluenza virus 2 strain Greer is propagated in LLC-MK2 Derivative cells (ATCC CCL-7.1). This strain was isolated in 1955 in Ohio from an 11-month-old human female with acute laryngotracheobronchitis. This product is whole-genome sequenced and has applications in respiratory disease research.
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A DNA sequence encoding the Influenza A virus (A/Wisconsin/588/2019 (H1N1)) hemagglutinin (EPI1661758) (Met1-Gly519, with a mutation Tyr108Phe), termed as HA, was expressed with a c-terminal AVI tagged polyhistidine tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
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Monothioglycerol MTG can be used as an alternative for 2-mercaptoethanol 2ME since it exhibits comparable performance to 2ME when it is added to a medium in the culture of mouse ES D3 strain cells This product is added to the culture medium as a reducing agent
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